The INEPT experiment is the most basic example of an experiment in the polarization transfer class of nmr experiments. Polarization transfer means that magnetization is transferred from one nucleus to another, usually from 13C to 1H.
First, the pulse sequence for the INEPT experiment:
The INEPT experiment suffers from the fact that the proton decoupler cannot be used during acquisition or the antiphase multiplets would collapse into nothing.If we add a waiting time at the end of the pulse sequence to allow for antiphase magnetization to rotate into phase (and use a pi pulse in the middle to get rid of chemical shift problems) we will then be able to use the decoupler. This will give us more sensitivity, always a good thing in nmr. Here is the modified INEPT pulse sequence INEPTR (Refocussed INEPT):
